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Si3N4  300kV  [001]   thickness = 5.2nm


Cs 1 mm -> 0

Cs  vs  df  and C5,   ( i.e. Scherzer focus,  and +C5 )

2-fold astigmatism

4-fold astigmatism

Star and 6-fold astigmatism

Coma and 3-fold astigmatism

 5-fold astigmatism,  3-lobe, 4th order coma 

Hemoglobin  300kV

Achromatic line : (analytical calculation vs numerical  calculation)

Spatial coherence reduced by the odd symmetric wave aberration 

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